Extended Data Fig. 1: LPS stimulation drives fumarate accumulation and protein succination.
From: Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
a-c, Fumarate-mediated protein succination with LPS (n = 3) and 2SC abundance in NS and LPS-stimulated BMDMs (n = 5; LPS 4 h). d, Heatmap of metabolites linked to aspartate-argininosuccinate shunt in NS and LPS-stimulated BMDMs (n = 5; LPS 24 h) e, Metabolite abundance of aspartate-argininosuccinate shunt metabolites in LPS-stimulated BMDMs pre-treated with DMSO or AOAA (n = 3; LPS 4 h; aspartate (P = 0.0000005)). f, Asl expression with silencing of Asl following LPS stimulation (n = 3; LPS 24 h). g, Fumarate levels with silencing of Asl following LPS stimulation (n = 3; LPS 24 h). c,e-g, Data are mean ± s.e.m. a, 1 representative blot of 3 shown. n = biological replicates. P values calculated using two-tailed Student’s t-test for paired comparisons or one-way ANOVA for multiple comparisons.
