Extended Data Fig. 3: LPS stimulation drives fumarate accumulation via glutamine anaplerosis and an aspartate-argininosuccinate shunt.
From: Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
a, Schematic diagram indicating 15N2-glutamine tracing into distinct metabolic modules. b, 15N2-glutamine tracing into glutamate and asparagine in LPS-treated BMDMs (m+1 and m+2 labelling intensity and total isotopologue fraction distribution) (n = 3; LPS 4 h). c, 15N2-glutamine tracing into GSH and GSSG in LPS-treated BMDMs (m+1 and m+2 labelling intensity and total isotopologue fraction distribution) (n = 3; LPS 4 h). d, 15N2-glutamine tracing into aspartate, arginine and citrulline in LPS-treated BMDMs (m+1 labelling intensity and total isotopologue fraction distribution) (n = 3; LPS 4 h; aspartate (P = 0.000001)). Data are mean ± s.e.m. n = biological replicates. P values calculated using one-way ANOVA for multiple comparisons.
