Extended Data Fig. 4: Increase in aspartate-argininosuccinate shunt metabolites in cytosol and Irg1–/– macrophages.
From: Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
Heatmap (min-max) of metabolites linked to mitochondrial bioenergetics and redox signalling (a) and the aspartate-argininosuccinate shunt (b) in NS and BMDMs (n = 3; LPS 24 h). c, Metabolite abundance of TCA cycle and aspartate-argininosuccinate shunt metabolites in WT and Irg1–/– BMDMs (n = 3; LPS 24 h); itaconate (P = 0.00000000000002, succinate (P = 0.00000003), fumarate (P = 0.000018)). d, Nitrite levels in WT and Irg1–/– BMDMs (n = 3; LPS 24 h). e, Schematic of metabolic changes occurring during mid-phase TCA cycle rewiring in WT and Irg1–/– BMDMs. Data are mean ± s.e.m. n = biological replicates. P values calculated using two-tailed Student’s t-test for paired comparisons or one-way ANOVA for multiple comparisons. Schematic in panel e was created using BioRender (https://biorender.com).
