Fig. 3: Integrity of the CTT is required for it to facilitate magnetosensitivity.
From: Essential elements of radical pair magnetosensitivity in Drosophila
a, Schematic of the domain structure of full-length DmCRY, including the CT (amino acids 491–542) and CTT (amino acids 521–542). The four Trp residues, presumed to be essential for the canonical RPM, are indicated by red asterisks. A putative PDZ-binding site (EEEV 528–531, shown in red) was mutated (Val531) DmCRY(V531K). The Trp residue (Trp536) mutated in Luc–CT(W536F) is shown in green. b, DmcryV531K expressed in clock neurons (tim-GAL4) does not support magnetosensitivity in the circadian period-shortening assay. A two-way ANOVA revealed no significant main effects or interaction effects (interaction, F1,52 = 0.09, P = 0.77). n = 53 (DD), n = 43 (BL pre-sham), n = 41 (BL + sham), n = 41 (BL pre-MF), n = 38 (BL + MF) (Extended Data Table 4a). c, Expression of DmcryV531K in aCC neurons is sufficient to support BL sensitivity (t9 = 2.934, P = 0.017, n = 10; Extended Data Fig. 7a) but not potentiation in the BL + MF condition (100 mT, t18 = 0.299, P = 0.768, n = 10). d, Expression of DmcryM, a truncated CRY variant lacking the terminal 19 amino acids, including the PDZ-binding motif (528–531), does not support sensitivity to a 300 µT MF (3 Hz, two-way ANOVA, F1,122 = 0.021, P = 0.89). n = 26 (DD), n = 31 (BL pre-sham), n = 31 (BL + sham), n = 32 (BL pre-MF), n = 32 (BL + MF) (Extended Data Table 4b). The blue asterisks represent significance values before versus during BL exposure (same cells, two-tailed paired t-tests) and the black asterisks represent comparisons of the BL versus BL + MF condition (different cells, unpaired two-tailed t-tests). The reported n value for each electrophysiological recording is derived from independent cells from biologically independent animals. The reported n values for each circadian period derives from biologically independent animals. Data are mean ± s.e.m.
