Extended Data Fig. 3: Supporting electrophysiological data (controls).

(a). Averaged data for parental (control) GAL4 and UAS genotypes separately in a Dmcry-null mutant background. Without the GAL4 and UAS elements combined the Dmcry-transgene (under UAS control) is not expressed and no significant BL or BL+MF response is seen. A 2-way ANOVA of the controls in both BL or BL+MF showed no significant interaction (F_(4,50) = 0.52, p = 0.719), n for all UAS-Dmcry transgenic controls in BL and BL+MF = 5. n for elav-GAL4; ; Dmcry⁰²/Dmcry⁰³ driver line BL and BL+MF = 10. (b-c). Raw AP counts (i) for each aCC neuron recorded in the 15 s before vs. the 15 s during BL or BL±MF exposure for expression of the respective UAS-Dmcry transgene stated (paired t-tests – two tailed). As an additional comparison of MF effect, unpaired t-tests – two tailed, were used to determine significant differences between raw AP counts for both ‘before’ exposure conditions (grey line), and for during BL vs. BL+MF exposure (purple line). The reported n for each electrophysiological recording is derived from independent cells from biologically independent animals. The reported n for each circadian period derives from biologically independent animals. Error bars denote ±SEM, ns p ≥ 0.06, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.