Fig. 2: PaMurA(C117S) is a potent activator of PaLpxC in vivo.
From: Coordination of bacterial cell wall and outer membrane biosynthesis
a, Viability assay in which serial dilutions of PAO1 harbouring an empty plasmid or the indicated PamurA variant under IPTG-inducible control were plated on LB agar with or without IPTG supplementation as indicated. Data are representative of three biological replicates. b, Ratio of LpxC product to substrate detected by liquid chromatography–mass spectrometry in the aqueous fraction of methanol–chloroform-extracted P. aeruginosa whole-cell lysates. Dots indicate the values obtained for three biological replicates, bars indicate the mean, and error bars represent their standard deviation. c, Model structure of the PaLpxC–PaMurA complex predicted by AlphaFold19. PaLpxC is represented in gold and PaMurA is represented in cyan. PaMurA residues G58 and E406 are highlighted in red and residue C117 is highlighted in navy. PaLpxC active-site residues34 are depicted in orange.
