Fig. 3: Direct interaction between LpxC and MurA is observed in diverse bacteria.
From: Coordination of bacterial cell wall and outer membrane biosynthesis
a, MurA–LpxC interaction scores calculated from a direct coupling analysis-based approach (Methods) plotted on a maximum-likelihood phylogenetic tree based on concatenated MurA and LpxC sequences generated with FastTree. Experimentally tested interactions are highlighted. b, Purified F–LpxC (2.5 µM) was mixed in a 1:1 ratio with purified H–MurA in the presence or absence of CHIR-090 (5.7 µM) as indicated. The mixtures were pulled down with anti-Flag resin and the input and eluate were subjected to SDS–PAGE and Coomassie staining. Mobilities of H–MurA and F–LpxC are indicated by a cyan or gold arrowhead, respectively. Data are representative of at least two replicates. For gel source data, see Supplementary Fig. 1.
