Extended Data Fig. 1: Regulation of LpxC in P. aeruginosa differs from that observed in E. coli.

(a) Anti-His immunoblot detecting H-^PaLpxC expressed from the native chromosomal locus in wild-type cells or an ftsH deletion mutant. A corresponding blot for RpoA was used as a loading control. Data are representative of 3 biological replicates. (b) Spot titer assay in which serial dilutions of PAO1 harboring an empty plasmid or one encoding His-^PalpxC or His-^EclpxC under arabinose-inducible control were plated on LB agar supplemented with arabinose and/or the LpxC inhibitor CHIR-090 as indicated. Plates were incubated at 37 °C for 20 h before being imaged. Data are representative of 3 biological replicates. (c) Anti-His immunoblot analysis of His-^PaLpxC or His-^EcLpxC protein levels in exponentially growing PAO1. Immunoblot for RpoA serves as a loading control. Data are representative of 3 biological replicates. For gel source data, see Supplementary Fig. 1.