Extended Data Fig. 3: MurA is essential and its depletion phenocopies simultaneous inhibition of PG and LPS biosynthesis.
From: Coordination of bacterial cell wall and outer membrane biosynthesis
(a–c) Growth curves of P. aeruginosa strains in LB with or without IPTG as indicated. Dots represent the average of 3 biological replicates and dashed lines indicate the standard deviation. The following strains were used: (a) PAO1 [WT] and PA1080 [∆PA4701], (b,c) PAO1 [WT], PA1118 [∆murA Plac-murA], and PA1135 [∆murB Plac-murB]. (d) Phase contrast images of P. aeruginosa cells after 1 hr treatment with the indicated antibiotic(s). Scale bar indicates 2 µm. Data are representative of at least 2 biological replicates (e) Phase contrast images of the indicated P. aeruginosa murA or murB depletion strains grown for 4 h in the presence or absence of inducer as indicated. MurB depletion was analyzed as a control to compare the phenotype of inactivating another early step in PG synthesis with that of MurA. Scale bar indicates 2 µm. Data are representative of at least 3 biological replicates (f) Quantification of cell width after 1 hr treatment with the indicated antibiotic(s) or after depletion of MurA or MurB. Each dot represents an individual cell and the median of the population is indicated by a black line. n indicates the number of cells analyzed. For each condition, the cells quantified were derived from a single population and data are representative of biological duplicates.
