Extended Data Fig. 9: Targeted siRNA screen of key genes involved in mtDNA release and regulation of mitochondrial morphology. | Nature

Extended Data Fig. 9: Targeted siRNA screen of key genes involved in mtDNA release and regulation of mitochondrial morphology.

From: Fumarate induces vesicular release of mtDNA to drive innate immunity

Extended Data Fig. 9: Targeted siRNA screen of key genes involved in mtDNA release and regulation of mitochondrial morphology.

a, Number of cytosolic DNA foci per cell in cFh1−/−CL1 cells treated with indicated siRNAs. n = 3 independent experiments. b-d, Quantification of mtDNA copy number by ddPCR using either a mtCo3 (b), ND1 (c) or D-loop (d) probe, from isolated cytosolic fractions of cFh1−/−CL1 cells. n = 3 independent experiments. e, Representative confocal images of cFh1+/+ and cFh1−/−CL1 cells. Mitochondria and cytochrome c (Cyt c) were labelled using anti-TOM20 and anti-cytochrome c antibodies, respectively. Scale bar: 10 μm. f, Immunoblots of specified proteins from whole cells (WCE), cytosol and heavy membrane (crude mitochondria) isolated fractions of cFh1 cells. Data are mean ± s.e.m. a-d, two-tailed unpaired t-test, p-values are indicated above each specific siRNA and relative to the scramble (scr) siRNA transfected control.

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