Extended Data Fig. 6: The photocycle of Kin4B8.
From: Phototrophy by antenna-containing rhodopsin pumps in aquatic environments
a, Chromatogram of HPLC analyses (left) and the compositions of the retinal isomers (right) in Kin4B8 without (top) and with (bottom) lutein under the dark (gray) and light-adapted (green) conditions. at, 11, 13, syn, and anti indicate all-trans, 11-cis, 13-cis, syn, and anti configurations, respectively. b, Two-dimensional plot of transient absorption change (left), transient absorption spectra at different time points (middle), and time course of the transient absorption change (right) of Kin4B8 without (top) and with (bottom) lutein. Peaks derived from the absorption change of lutein are indicated by an asterisk. c, Absorption spectra of the photointermediates of Kin4B8 without (left) and with (right) lutein. Peaks derived from the absorption change of lutein are indicated by asterisks. d, Photocycle model of Kin4B8. Conformational change of rhodopsin affects the structure of lutein (blue arrow) from K3/M1 to O4/Kin4B8′2. e, The ratios of transient absorption change in Kin4B8 with and without lutein at different excitation wavelengths (415, 432, 457, 473, 487, 552, and 601 nm) (bars colored according to the color of excitation light). The absorption spectra of Kin4B8 without (pink line) and with (orange line) lutein were overlaid. The red dashed line indicates no difference between without and with lutein.
