Extended Data Fig. 7: Light-induced proton-pumping activity of Kin4B8 and Kin4B8-G153F.
From: Phototrophy by antenna-containing rhodopsin pumps in aquatic environments
a and b, Monitoring of pH changes in E. coli spheroplasts suspension expressing Kin4B8 or Kin4B8-G153F, respectively, with and without zeaxanthin. The spheroplasts were illuminated with violet (430 nm), blue (450 nm), or green (550 nm) light for 2 min (indicated by the colored bars). An enlarged plot of the first 15 s of illumination is displayed to the right of each measurement. The presented traces are the average of six or more independent biological replicates (Error bars represent the SEM).
