Extended Data Fig. 4: Cytotoxicity induced by neoantigen-specific TCRs from patient 1 upon co-culture with the autologous cell line.
From: Neoantigen-targeted CD8+ T cell responses with PD-1 blockade therapy
Healthy donor T cells genetically engineered to express the captured neoTCRs from patient 1 were co-cultured with the autologous (M495) or a mismatched cell line (M202). a-b, Specific target-cell killing by neoTCR gene-edited T cells of the autologous cell line and the mismatched control (P:T ratio 5:1, n = 4)). Melanoma cell lines were pre-treated with media with IFNγ 24 h prior co-culture with T cells. The plots are divided between TCRs without killing activity (a) and TCRs with intermediate killing (b). * p < 0.05 vs Neo12, two-tailed unpaired t test with Holm-Sidak adjustment for multiple comparison (n) indicates the number of biological replicates. Exact p-values provided in Supplementary Information. Mean and individual values are shown. All T-cell products contain CD8+ and CD4+ gene-edited T cells.
