Extended Data Fig. 7: IFN-γ in the T cell population and microglia can directly present antigen to CD8+ T cells in vitro. | Nature

Extended Data Fig. 7: IFN-γ in the T cell population and microglia can directly present antigen to CD8+ T cells in vitro.

From: Microglia-mediated T cell infiltration drives neurodegeneration in tauopathy

Extended Data Fig. 7: IFN-γ in the T cell population and microglia can directly present antigen to CD8+ T cells in vitro.The alternative text for this image may have been generated using AI.

(a) Ligand-receptor analysis in T cells and microglia. (b) IFN-γ expression in brain parenchyma (CD45Total) 12 cell types and T cells from brain parenchyma 15 clusters of T cells as visualized by UMAP plot. (c) The gating strategy for sorting naïve OT-1 CD8+ T cells, dendritic cells (DCs), and microglia. (d) Representative flow cytometry plot to assess the proliferation of OT-1 T cells by cell tracer violet (CTV) dilution after 3 days of co-culture with APCs in the presence of OVA. (e) Representative flow cytometry plot showing dose dependent OVA antigen presentation by DCs, microglia, or microglia in the presence of IFNγ assayed by OT-1 proliferation. (f) Percent of proliferating OT-1 T cells under the indicated conditions. Data are from one representative experiment. Two independent experiments were done showing similar results.

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