Extended Data Fig. 3: T cell infiltration in the brain parenchyma with significant tauopathy.
From: Microglia-mediated T cell infiltration drives neurodegeneration in tauopathy
(a) Representative flow cytometry gating plot of splenic lymphocytes. (b) Quantification of the proportion of indicated lymphocytes and their subsets among 9.5-month E4, A/PE4, and TE4 mice. (E4: n = 4, A/PE4: n = 4, TE4: n = 8). p = 0.648, 0.492, 0.992 for E4 vs. A/PE4; E4 vs. TE4; A/PE4 vs. TE4 in T cells; p = 0.614, 0.518, 0.089 for E4 vs. A/PE4; E4 vs. TE4; A/PE4 vs. TE4 in CD4+ T cells; p = 0.665, 0.719, 0.196 for E4 vs. A/PE4; E4 vs. TE4; A/PE4 vs. TE4 in CD8+ T cells. Two-way ANOVA with Tukey’s post hoc test. p = 0.629, 0.472, 0.095 for E4 vs. A/PE4; E4 vs. TE4; A/PE4 vs. TE4 in Treg. One-way ANOVA with Tukey’s post hoc test. (c) Quantification of CD3+ T cell number per DG area with 0.3 mm2 in 9.5 month E3, TE3, E4, TE4 mice. (E3: n = 5, TE3: n = 6, E4: n = 15 and TE4: n = 13). Two-way ANOVA with Tukey’s post hoc test. p = 0.1342 for TE3 vs. TE4. (d) Representative images of 9.5-month old E4, TE4, A/PE4 and 5xE4 mouse brain sections stained with CD3, Iba1, Aβ and X-34. Scale bar = 20 μm. Images are representative of results from n = 4 in E4, A/PE4 and TE4 respectively. (e) TEM image demonstrating presence of a cell with T cell like features in brain parenchyma of 9.5 month of TE4 mouse. Scale bar = 2 μm. Images are representative of results from n = 3 in TE4 mice.
