Fig. 1: WGD induces LCS.
From: Whole-genome doubling drives oncogenic loss of chromatin segregation
a, Schematic representation of the WGD and CIN induction experimental approaches in the indicated cell lines. DCB, dihydrocytochalasin B; Noc, nocodazole. b, Representative images of metaphase spreads for CP-A TP53−/− clone 3 (C3), RPE TP53−/− and K562 cell lines. c, Quantification of chromosomes per cell for CP-A C3 TP53−/−, RPE TP53−/− and K562 cell lines. The number of cells is indicated. For the violin plots, dashed line is the median, dotted lines are quartiles. d, Heatmap of the ratios of interchromosomal contact enrichments (observed versus expected) between WGD and control samples for CP-A TP53−/− cells, RPE TP53−/− cells and K562 cells and between RPE TP53−/− CIN-only cells and control cells. Chromosomes were sorted by length. e, Heatmap of ratios of genomic bins belonging to the indicated subcompartments that gain or lose contacts in the indicated conditions. f, Boundary insulation scores in control cells and WGD or CIN-only cells in the indicated cell lines for the shared top insulating boundaries. For f–h, P values were calculated using two-tailed Wilcoxon test.
