Extended Data Fig. 6: Manipulation of leg-joint movement.
From: Molecular sensing of mechano- and ligand-dependent adhesion GPCR dissociation
a, Position of femorotibial joint in adult metathoracic leg. Adapted from ref. 67. b,c, Adult flies were glued to a support and videotaped before, during and after the leg immobilization procedure. In the photographs the fly is displayed only during the immobilization interval, when the experimental metathoracic leg is fixed in extension with a taut restraint during the leg extension (b) and flexion (c) phases. The support plate fixation point of the restraint is not depicted in the images. The contralateral leg was allowed to move freely during all intervals of the procedure. Dashed lines indicate axes of the femur and tibia, between which the angle was determined for the immobilized and mobile leg, respectively. d, The motion range of the joint (Δα) was determined by measuring the difference between the femoro-tibia axes angle during maximal extension (αe) and flexion (αf). For clarity axes of mobile leg as shown in b,c were mirrored in the illustration.
