Extended Data Fig. 4: Comparison of Cirl-NRS activity with different binary expression system readouts. | Nature

Extended Data Fig. 4: Comparison of Cirl-NRS activity with different binary expression system readouts.

From: Molecular sensing of mechano- and ligand-dependent adhesion GPCR dissociation

Extended Data Fig. 4: Comparison of Cirl-NRS activity with different binary expression system readouts.The alternative text for this image may have been generated using AI.

a, Organization of the Cirl locus, Cirl-NRS alleles and their gene products. b–d, Cirl-NRS-LexA (b), Cirl-NRS-GAL4 (c) and Cirl-NRS-QF2 (d) sensors display comparable activity in adults in neurons of the proboscis (chevron), eyes (double chevron) and leg joints (arrowheads). Reporter transgene are: 13xLexAop2-6xmCherry-HA (b), 20xUAS-6xmCherry-3xHA (c) and QUAS-mtdTomato-3xHA (d). Scale bars = 0.5 mm. e–g, Cirl-NRS dissociation signals reported using the (e) LexA/lexAop, (f) GAL4/UAS and (g) QF2/QUAS binary expression systems. Top panels show Cirl-NRS activity in the eyes (double chevrons), proboscis (chevrons), and the pedicel (white arrows) and funiculus (grey arrows) of the antenna. Middle panels show Cirl-NRS activity in the leg, bottom panels show a close-up of the femorotibial joint with Cirl-NRS-positive mechanosensory neurons (white arrowheads). Scale bars = 250 µm (heads and legs), 50 µm (joints). All experiments independently repeated 3x with similar results.

Back to article page