Extended Data Fig. 7: Serine-to-leucine mistranslation of TCT codons in Ec_Syn61∆3-SL cells.
From: A swapped genetic code prevents viral infections and gene transfer
a) MS/MS spectrum of the serine-to-leucine mistranslated TufA peptide. b) MS/MS spectrum of the wild-type TufA peptide. The figure shows the amino acid sequence and MS/MS spectrum of the Ec_Syn61∆3-SL-expressed TufA protein fragment, together with its genomic sequence, in which the serine-coding TCT codon (as shown in panel b) is partially mistranslated as leucine (as shown in panel a). The experiment was performed by analyzing the total proteome of Ec_Syn61∆3-SL cells, expressing Leu9-tRNAYGA from Escherichia phage OSYSP (GenBank ID MF402939.1) by tandem mass spectrometry (Methods). MS/MS data was collected once.
