Extended Data Fig. 1: Viral serine tRNAs decode TCA codons as serine, but Syn61∆3 obstructs the replication of viruses containing genomic tRNA-SerUGA.
From: A swapped genetic code prevents viral infections and gene transfer
a) Viral TCR suppressor tRNAs decode TCA codons as serine. The amino acid identity of the translated TCR codon within elastin16 TCA-sfGFP-His6 was confirmed by tandem mass spectrometry from Syn61∆3 expressing the tRNA-SerUGA of Escherichia phage IrisVonRoten (GenBank ID MZ501075)24. The figure shows the amino acid sequence and MS/MS spectrum of the analyzed elastin16 TCA peptide. MS/MS data was collected once. b) Syn61∆3 obstructs the replication of viruses containing genomic tRNA-SerUGA. Figure shows the titer of twelve tRNA gene-containing coliphages24, after 24 h of growth on MDS42 and Syn61∆3. All analyzed bacteriophages, except MZ501058, contain a genomic tRNA-SerUGA tRNA that provides TCR suppressor activity based on our screen (Fig. 1b, Supplementary Data 1). Early exponential phase cultures of MDS42 and Syn61∆3 were infected at an MOI of 0.001 with the corresponding phages, and free phage titers were determined after 24 h of incubation. Measurements were performed in n = 3 independent experiments (i.e., MDS42 + MZ501058, MZ501065, MZ501075, MZ501105, MZ501106; Syn61∆3 + MZ501046, MZ501067, MZ501066, MZ501096, MZ501074, MZ501098) or in n = 2 independent experiments (i.e., MDS42 + MZ501046, MZ501066, MZ501067, MZ501074, MZ501089, MZ501096, MZ501098; Syn61∆3 + MZ501058, MZ501065, MZ501075, MZ501089, MZ501105, MZ501106); dashed line represents input phage titer without bacterial cells (i.e., a titer of 420 PFU/ml); dots represent data from independent experiments; bar graphs represent the mean; error bars represent the SEM based on n = 3 independent experiments.
