Extended Data Fig. 5: Nucleotide-sensitivity of CFTR dynamics.
From: CFTR function, pathology and pharmacology at single-molecule resolution
a. Representative ATP titration of CFTR-mediated current in an inside-out excised patch. C-terminally GFP-fused wild-type CFTR was phosphorylated prior to the recording. b. FRET distributions of phosphorylated CFTRFRET at the indicated ATP concentrations. c-d. Dwell time distributions of low FRET (c) and high FRET (d) states for phosphorylated CFTRFRET at the indicated ATP concentrations. e. Contour plots for phosphorylated wild-type and E1371Q variant CFTRFRET at the indicated ATP concentrations. f. Transition density plots for the same conditions as in e.
