Extended Data Fig. 6: Quaternary structure assignment of Vs.4.
From: Ubiquitin-like conjugation by bacterial cGAS enhances anti-phage defence
a, schematic representation of crystal packing and unit cell of Vs.4. Monomers of Vs.4 cluster in hexamers in the lattice. To illustrate hexamer clusters, each hexameric complex of Vs.4 is highlighted in a unique color. b, PISA was used to analyze the Vs.4 structure for possible higher-order assemblies of Vs.4. In total, 5 assemblies were predicted. The top prediction (top left) is the only hexameric assembly that was predicted to be stable (see Methods). c, analytical ultracentricugation of Vs.4 bound to cGAMP. Vs.4 forms a hexaxamer that is stable on the time scale of the sedimentation experiment at 45 μM and 15 μM with a sedimentation coefficient of 4.64 and a molecular weight >50.3 kDa (61.8 kDa expected for hexamer).
