Extended Data Fig. 8: Transcription factors active in extravillous trophoblast cell states (all donors).
From: Spatial multiomics map of trophoblast development in early pregnancy
a: Heatmap showing z-score of normalised, log-transformed and variance-scaled expression of transcription factors (TFs) upregulated during trophoblast invasion in all donors (n = 17 donors with trophoblast present). Y-axis indicates cell state, X-axis lists TFs. Differential expression (upregulated genes) is tested along invading trophoblast trajectory (as shown in Fig. 2a) in a retrograde manner using limma approach (FDR < 0.05, with Bonferroni correction for multiple hypotheses testing. b: Dot plot showing normalised, log-transformed and variance-scaled expression of genes (X-axis) of signalling molecules upregulated in EVT (Y-axis) in all donors (n = 17 donors with trophoblast present). c: Heatmap showing z-score of normalised, log-transformed and scaled expression of TFs downregulated during trophoblast invasion in all donors (n = 17 donors with trophoblast present). Y-axis indicates cell state, X-axis lists TFs. Differential expression (downregulated genes) is tested along invading trophoblast trajectory (as shown in Fig. 2a) in a retrograde manner using limma approach (FDR < 0.05, with Bonferroni correction for multiple hypotheses testing). d: Schematic representation of signalling pathways in distinct spatial microenvironments. Villous cytotrophoblast (VCT), cytotrophoblast cell column (CCC), extravillous trophoblast (EVT), interstitial EVTs (iEVTs), giant cells (GC), endovascular EVT (eEVT), microenvironment (ME), transcription factors (TFs).
