Extended Data Fig. 8: Normal tissue study design and ddPCR results.

A) Schematic indicating normal lung tissue cohorts analysed by ddPCR and Duplex-seq. B) TRACERx and PEACE Cohort for ddPCR of 5 EGFR mutations. (i) Clinical information for each patient, (ii) Tumour EGFR mutation status, (iii) Normal EGFR mutation status. C) Representative H & E images from anthracotic pigment identification in TRACERx normal tissue. D) Comparing area of normal tissue harbouring anthracotic pigment in never smokers (n = 43) and smokers (n = 138). Each dot represents the ratio of pigmented area respective to total tissue in each anthracosis positive normal lung tissue sample. Two-sided Wilcox test p-value is reported. E) Regression analysis of characteristics influences EGFR mutant (EGFRm) presence in normal lung tissue for ddPCR-TRACERx cohort (n = 195). The illustrations in a were created using BioRender (https://biorender.com).