Extended Data Fig. 4: OAT is enriched in human and mouse PDA tumors.
From: Ornithine aminotransferase supports polyamine synthesis in pancreatic cancer
a, Gene expression analysis showing higher mRNA levels of OAT but not ALDH18A1 (aldehyde dehydrogenase 18 family member A1), ARG2 or ODC1 in human tumors compared to normal tissues. Data were derived from TCGA and GTEx datasets and represent 5 cancer types including pancreatic adenocarcinoma (PAAD), breast carcinoma (BRCA), lung, colon and prostate adenocarcinomas (LUAD, COAD and PRAD, respectively). TPM, transcript per million. T, tumor and N, normal tissue. Number of tissue samples n is indicated at bottom of panel. Box plots represent the interquartile range of data with the middle line being the median and whiskers spanning the minimum and maximum values. Plots and statistics were generated by GEPIA56 software. *p < 0.01 (one-way ANOVA). b, mRNA levels of genes in a quantified by qPCR, in 20 human cancer cell lines and 3 tissue-matched normal cell lines from the panel described in Fig. 1b, c. Ct indicates cycle threshold for each gene in the normal cell lines and is inversely proportional to mRNA levels. Data represent the mean of 3 technical replicates and are representative of two independent experiments. c, Representative immunohistochemical staining of OAT in PDA tumors and normal pancreas of tumor-bearing iKrasG12D or non-tumor-bearing iKrasWT mice treated with Dox (2g l−1 drinking water) for 3 weeks. Scale bar: 100 μm. d, Representative immunohistochemical OAT staining in human PDA, pancreatic intraepithelial neoplasia (PanIn) and adjacent normal cells in whole tissue sections of resected tumors from 4 different patients (Cases 1-4). Scale bar: 100 μm. In c,d, framed top corner insets represent a 5-fold magnification of the area delineated by a dashed square; data are representative of 2 independent experiments. e, H scores of OAT staining in PDA tumors and patient-matched normal pancreatic tissue performed on tissue microarrays (TMAs) of resected tumors from n = 31 patients. Data represent the mean ± s.d and p = 0.0003 was obtained by paired two-tailed t-test.
