Extended Data Fig. 9: Sub-nm RESI measurements.
a, DNA origami featuring six alignment strands (green R4) and six pairs of orthogonal docking strands (red R1, blue R3) spaced one base pair apart. b, RESI representation with RESI-localizations from round 1 in red and round 2 in blue illustrates excellent alignment. The distances between RESI-localizations from round 1 and 2 are defined as illustrated. c, Overlaying 42 DNA origami and performing a particle average recovers the structure with an alignment uncertainty of 1.2 Å CI = [0, 4.6] Å, showing distances between the average positions of the sites at 9.5 ± 2.6 Å (mean over six distances in the average ± mean over the error-propagated uncertainties of the six distances). Same scale applies to all magnification panels. CI describes 68% confidence interval.
