Extended Data Fig. 12: RESI reveals higher order arrangement of CD20 dimers in Rituximab-treated CHO cells.
a, DNA-PAINT imaging of whole mEGFP-CD20-expressing CHO cells, labeled with anti-GFP-nanobodies, shows clustered CD20- molecules in Rituximab-treated cells for three independent experiments. b, Zoom-in regions of DNA-PAINT show mEGFP- CD20 clustered into chain-like arrangements. c, RESI reveals sub-10-nm spaced receptor pairs within the clusters, unresolvable by DNA-PAINT. d, Whole-cell analysis of first nearest neighbor distances (1st NNDs) of CD20 receptors bound to Rituximab (histograms of the distances are displayed). Only RESI, but not DNA-PAINT, allows the routine detection of sub-10-nm distances between proteins. e, Routine detection of sub-10-nm distances by RESI recapitulates the first NND measured in the untreated case. Notably the NND peaks measured in the three repeats are consistent, independently of the protein density.
