Extended Data Fig. 13: Comparison of Rituximab treated CD20 data to simulated CD20 hexamer arrangements.

a, Example of ground truth simulated CD20 hexamers (light blue circles, simulated as triangles of dimers with intra-dimer distances of 13.5 nm as measured experimentally) with random distribution and orientation on a 2D surface at the experimentally determined density. b, Label uncertainty and labeling efficiency (black circles indicate labeled molecules) are taken into account in the simulation for a realistic comparison. c, Simulated proteins in hexameric arrangements represented as gaussians. d, Hexamers after DBSCAN cluster analysis (colors indicate clusters). e, RESI image of CD20 data after RTX-treatment. f, RESI-localizations of CD20 data after DBSCAN cluster analysis (colors indicate clusters). g, Number of molecules per detected cluster for the experimental data and the simulated hexamers. h, Circularity metric of experimental data and the simulated hexamers after convex hull analysis of the clusters. We note that the sharp drop at 0.605 stems from the maximum circularity metric for clusters where the convex hull is defined by three molecules. Notably, the absence of a circularity peak at ~0.7 in the experimental data suggests that CD20 molecules are not arranged in isolated ring-like hexameric structures.