Extended Data Fig. 1: RESI resolution estimation.
a, A grid of defined positions of binding sites is generated (top left), SMLM (DNA-PAINT) localizations are simulated as samples from a gaussian distribution (top right). Localizations for only one binding site were plotted for clarity. For each binding site, subsets of K localizations are randomly selected (bottom left) and averaged (bottom right). One exemplary subset and its average is highlighted. b, Resulting RESI-localizations are histogrammed to produce images at different resolutions (K values). c, RESI-localization precision σRESI vs K. Analytical dependence on \(\sqrt{K}\) (blue line) and numerical results (black dots). A total of 1200 SMLM localizations per site are simulated. Error bars represent mean ± 1 s.d.
