Extended Data Fig. 9: New McSCs become Oca2+ at each hair cycle.
From: Dedifferentiation maintains melanocyte stem cells in a dynamic niche
(a) Experimental scheme of TAM injection during one hair cycle (single TAM) or several continuous hair cycles (repeated TAM) in Oca2-CreER;Rosa-LSL-tdTomato mice. (b-c) Z-stack images of trace-labeled Tom+ cells in 3rd telogen HFs after single (b) or repeated (c) TAM injections. (d) Number of HFs containing Tom+ cells in HG after single or multiple TAM injections. HFs were counted at 1st telogen (green), 2nd telogen (blue) and 3rd telogen (orange). N = 3 mice, with at least 25 HFs analyzed per mice per time point. Data are represented as mean ± SD. P-value by two-way ANOVA with Bonferroni multiple comparison test. 95% confidence interval: −94.18 to −6.799. (e) Number of Tomato+ McSC per hair follicle from single and multiple TAM-injected mice at 3rd telogen. N = 3 mice with at least 3 HFs containing Tom+ cells analyzed per mice. Data are represented as mean ± SD. P-value by two-tailed unpaired t test. (f) Z-stack image of Tomato and Dct co-immunofluorescence at 3rd telogen following repeated TAM injections. To show the co-localization of green and red signals in all McSCs throughout the z-stack, the right top panels show the z-stack of first 15 stacks and the right bottom panels show the z-stack of last 15 stacks. Shown are representative images of 3 mice. (g) Schematic summary. Dashed white lines outline epithelial-dermal boundary. Scale bar: 20 μm. TAM: tamoxifen; d: day.
