Extended Data Fig. 13: Increased scattering of McSCs to the bulge in aged Dct-LacZ and Oca2-CreER;Rosa-LSL-tdTomato mice.
From: Dedifferentiation maintains melanocyte stem cells in a dynamic niche
(a) Experimental scheme of repeated plucking of Dct-LacZ mice. (b) Images of hair follicles from Dct-LacZ mice at 2nd telogen and 7th telogen after hair plucking. (c) Experimental scheme: early repeated plucking followed by natural aging of Oca2-CreER;Rosa-LSL-tdTomato mice. (d) Z-stack images of hair follicles from Oca2-CreER;Rosa-LSL-tdTomato mice at 3 months old (3rd telogen) and 14 months old telogen. (e) Quantification of Tom+ McSCs residing in the HG or bulge in Oca2-CreER;Rosa-LSL-tdTomato mice at indicated time points. N = 42 HFs from 2 mice at 3 months. N = 54 HFs from 2 mice at 14 months. Dashed lines outline epithelial-dermal boundary. Solid circles suggest location of dermal papilla. Scale bar: 20 µm. HG: hair germ; Bg: bulge; TAM: tamoxifen; d: day; PN: postnatal; Telo: telogen.
