Extended Data Fig. 3: Effect of pH on export of glucosinolates by UMAMIT29.
From: Export of defensive glucosinolates is key for their accumulation in seeds
Exported 2Propenyl glucosinolate (2Prop) (a), 4-methylthiobutyl glucosinolate (4MTB) (b) and indolyl-3-methyl glucosinolate (I3M) (c) levels were quantified in UMAMIT29-expressing oocytes (UT29, mean ± s.d. n = 10) or H2O-injected oocytes (mean ± s.d. n = 5) incubated at pH 5.5 or pH 7.4 for 4 h after injection of an equimolar mixture of glucosinolates (initial intracellular concentrations of individual glucosinolates are 50 µM). Glucosinolate export by UT29 at pH 5.5 and pH 7.4 was compared by one-way ANOVA analysis followed by Tukey’s post-hoc HSD test. Bars labelled with different letters are significantly different (P < 0.05).
