Extended Data Fig. 13: Locomotion suppresses spontaneous GABAergic signalling in layer 1 neurons but enhances sensory-evoked GABAergic signals.
From: Cortico-cortical feedback engages active dendrites in visual cortex
a, Expression and excitation strategy for dual-colour imaging. b, Average iGABA-SnFR fluorescence (top) and jRGECO1a fluorescence (bottom) in a typical field-of-view (300 μm). Visual stimuli were 20° or full field sinusoidal gratings and they produced qualitatively similar results. c, Example raw trace of iGABA-SnFR fluorescence recorded over the entire field of view and averaged over two imaging planes in layer 1. d, Mean GABA fluorescence over entire field-of-view during stationary and locomotion periods, averaged over trials in one session. e, Mean GABA fluorescence over entire field-of-view during stationary and locomotion periods, averaged across n = 33 sessions. f, Mean fluorescence during stationarity vs locomotion. Top, GABA fluorescence during baseline periods significantly decreased with locomotion (t-test, p = 5.5 x 10−5). Bottom, stimulus evoked (baseline-subtracted) GABA fluorescence significantly increased with locomotion (t-test, p = 3 x 10−7).
