Extended Data Fig. 4: Apical tuft dendrite imaging experiments.
From: Cortico-cortical feedback engages active dendrites in visual cortex
a, Example illustrating experimental workflow, which consisted of finding a sparsely labelled group of neurons, mapping visual receptive fields, tracing from the soma to the apical tuft while imaging at 800 nm and then finding a branch that was relatively uninterrupted by other processes before displaying visual stimuli relative to the known receptive field. b, Visual stimuli used in this experiment. c, Two additional examples of dendrites that exhibited independent events. Bottom example illustrates challenges to automated detection. Event #1 was included in our data as an independent event that clearly involved at least two distinct spines and the branch. Event #2 was similar to #1, but only one spine and the branch were visibly activated during this smaller magnitude event. Event #3 was a global event that happened to be larger magnitude in the distal ROI in comparison to the proximal ROI.
