Extended Data Fig. 7: Loss of astrocytic Gabbr1 disrupts cortical circuit function.
From: Inhibitory input directs astrocyte morphogenesis through glial GABABR
a. Schematic of viral labelling of inhibitory neurons and experimental timeline. b-e. Representative traces of spontaneous EPSCs and IPSCs from excitatory and inhibitory neurons from cortex of Gabbr1-cKO and controls. Associated cumulative and bar plots demonstrate quantification of sEPSC and sIPSC from 3 pairs of animals (b, n = 13, 15 cells; Kolmogorov-Smirnov test, **** P < 0.0001; two-tailed Mann-Whitney test, P = 0.8207, **P = 0.003; c, n = 15, 12 cells; Kolmogorov-Smirnov test, **** P < 0.0001; two-tailed Mann-Whitney test, P = 0.1995, 0.5888; d, n = 13, 15 cells; Kolmogorov-Smirnov test, **** P < 0.0001; two-tailed Mann-Whitney test, P = 0.7856, 0.0504; e, n = 11, 9 cells; Kolmogorov-Smirnov test, **** P < 0.0001; two-tailed Mann-Whitney test, P = 0.2299, 0.3796). f. Experimental timeline for behavioural analysis. g. 3-chamber social interaction and pre-pulse inhibition studies on Gabbr1-cKO and control mice from 10 animals in control group and 11 animals in cKO group. (left, GLME model with Sidak’s multiple comparisons test, *P = 0.015; right, two-tailed Mann-Whitney test, *P = 0.043). h-m. Summary of behavioural assays conducted on Gabbr1-cKO and control animals including open field (h), elevated plus maze (i), rotarod (j), parallel foot fall (k), contextual fear conditioning (l), and cued fear conditioning (m) on Gabbr1-cKO and control mice from 10 animals in control group and 11 animals in cKO group (two-tailed Mann-Whitney test, ***P = 0.0004) Data represent mean ± s.e.m. (b-e), s.d. (g-m).
