Extended Data Fig. 9: Analysis of astrocyte development in the Sox9-cKO and Nfia-cKO mouse lines.

a. Homer transcription factor motif analysis on differentially expressed genes (DEGs) from P1 and P14 timepoints from astrocytes isolated from the cortex, hippocampus, and olfactory bulb. b. Analysis of NFIA expression in Aldh1l1-GFP astrocytes from the Nfia-cKO and control at P7 in the cortex, hippocampus, and olfactory bulb; quantification of knockout efficiency was derived from 3 pairs of animals (two-way ANOVA, ****P < 0.0001). c. Analysis of SOX9 expression in Aldh1l1-GFP astrocytes from the Sox9-cKO and control at P7 in the cortex, hippocampus, and olfactory bulb; quantification of knockout efficiency was derived from 3 pairs of animals (two-way ANOVA, ****P < 0.0001). d. Analysis of NFIA expression in Aldh1l1-GFP astrocytes from the Nfia-cKO and control at P28 in the cortex, hippocampus, cerebellum, and olfactory bulb; quantification of knockout efficiency was derived from 3 pairs of animals (two-way ANOVA, ****P < 0.0001). e. Analysis of SOX9 expression in Aldh1l1-GFP astrocytes from the Sox9-cKO and control at P28 in the cortex, hippocampus, cerebellum, and olfactory bulb; quantification of knockout efficiency was derived from 3 pairs of animals (two-way ANOVA, ****P < 0.0001, *P = 0.0205). f. AAV-based overexpression of NFIA in the developing cortex, analysis of Gabbr1 expression at P28 via RNAscope; n = 3 pairs of animals (19, 18 cells; LME model, *P = 0.023, ***P = 0.00034). g. AAV-based overexpression of SOX9 in the developing olfactory bulb, analysis of Gabbr1 expression at P28 via RNAscope; n = 3 pairs of animals (20,25 cells). h. Chromatin immunoprecipitation of NFIA from P28 cortex or SOX9 from P28 olfactory bulb (OB), followed by PCR detection of association with motif in proximal promoter region of Gabbr1. Scale bars, 50 μm (b–e), 10 μm (f–g). Data represent mean ± s.d. (b–e), median, minimum value, maximum value and IQR (f–g).

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