Extended Data Fig. 11: Electrophysiological and behavioural analysis of the Nfia-cKO mouse line.
From: Inhibitory input directs astrocyte morphogenesis through glial GABABR
a. Schematic of synaptic markers and cortical layers. b–c. Antibody staining for makers of excitatory synapses Vglut1–PSD95 (b) and Vglut2–PSD95 (c) in layer I of the cortex from Nfia-cKO or control mice at P28, quantification is derived from 3 pairs of animals (GLME model). d. Antibody staining for markers of inhibitory synapses VGAT–Gephyrin at P28; quantification is derived from 3 pairs of animals (GLME model). e–f. Representative traces of spontaneous EPSCs and IPSCs from excitatory (e) and inhibitory (f) neurons from cortex of Nfia-cKO and controls. Associated cumulative and bar plots demonstrate quantification of amplitude and frequency of sEPSC and sIPSC from 3 pairs of animals (e, Kolmogorov-Smirnov test, **** P < 0.0001, *P = 0.0181, two-tailed Mann-Whitney test; f, Kolmogorov-Smirnov test, **P = 0.0026, ****P < 0.0001, two-tailed Mann-Whitney test, *P = 0.0149). g–h. Representative traces of action potential in layer II/III neurons upon varying injected current in Nfia-cKO and control. Summary data of action potential firing, resting membrane potential, and threshold from excitatory neurons (g) and inhibitory neurons (h); quantification is derived from at least 3 pairs of animals (two-way ANOVA and two-tailed Mann-Whitney test). i-j. 3-chamber social interaction and prepulse inhibition behavioural studies on Nfia-cKO and control mice from 11-13 animals in each group (i, n = 13 pairs of animals, two-way ANOVA, ***P = 0.0002; j, n = 13, 11 animals, two-tailed Mann-Whitney test, **P = 0.0050). k–p. Summary of behavioural tests from NFIA-cKO and control, including open field; n = 13, 11 animals (k), elevated plus maze; n = 10 animals (l), rotarod; n = 5, 8 animals (m), parallel footfall; n = 13, 11 animals (n), contextual fear conditioning; n = 12, 10 animals (o), and cued fear conditioning; n = 12, 10 animals (p) (two-tailed Mann-Whitney test, *P = 0.0265, 0.0136). Scale bars, 3 μm (b–d). Data represent mean ± s.d. (b–d, i, o–p), mean ± s.e.m. (e–h, j–n).
