Extended Data Fig. 1: Normalization of astrocyte morphology in the adult after developmental activation of inhibitory neurons.
From: Inhibitory input directs astrocyte morphogenesis through glial GABABR
a-b. Analysis of SOX9 (e) and Ki67(f) expression within Aldh1l1-GFP astrocytes at P21 after activation of inhibitory neurons (or control); quantification was derived from n = 3 pairs of animals (a, 20,24 images; GLME; b, 12, 11 images; GLME). c. CNO only treatment of Aldh1l1-GFP mice from P7-P21 and analysis of astrocyte morphology at P21. n = 3 animals (39 cells; GLME model with Sidak’s multiple comparisons test). d. Heatmap depicting expression of GABA receptor subunits in developing astrocytes from the cortex (CX), hippocampus (HC), or olfactory bulb (OB) at P1, P7, and P14. See Extended Data Figure 2. d. Example of gating strategy and percentage of GFP+ astrocytes FACS isolated from P1 animal. e. Heatmap depicting expression of GABA receptor subunits in developing astrocytes from the cortex (CX), hippocampus (HC), or olfactory bulb (OB) at P1, P7, and P14. See Extended Data Figure 2. f. Schematic of DREADD-based activation of inhibitory neurons in post-natal Aldh1l1-GFP mice and experimental timeline. g. Imaging of P60 Aldh1l1-GFP astrocytes after hM3Dq activation of inhibitory neurons; quantification of morphological complexity using Sholl analysis, branch number, and total processes at P21; n = 3 pairs of animals (26,35 cells; upper, GLME model with Sidak’s multiple comparisons test; bottom, GLME model). Scale bars, 20 μm (a–c), 30 μm (g). Data represent mean ± s.d. (a–c, g upper), median, minimum value, maximum value and IQR (g bottom).
