Extended Data Fig. 2: Transcriptomic RNA-Sequencing analysis of developing astrocytes in the cortex, hippocampus, and olfactory bulb at P1, P7, and P14.
From: Inhibitory input directs astrocyte morphogenesis through glial GABABR
a. Heatmap depicting the expression of neuron-specific and astrocyte-specific genes from P1, P7, and P14 FACS isolated Aldh1l1-GFP astrocytes from the listed brain regions. b. Aldh1l1-GFP astrocytes from the cortex at P1, P7, P14. Principal component (PC) analysis against top 2,000 variable genes across the region and timepoints examined from 3-4 animals in each group. c. Heatmap depicting differential patterns of gene expression in developing astrocytes across brain regions and timepoints. d. Gene Ontology (GO) analysis of the common and region-specific patterns of gene expression. e–f. Ald1l1-CreER; ROSA-LSL-tdTomato mouse line treated with tamoxifen at P1, harvested at P28. Co-immunostaining of tdTomato labelled cells with Sox9, Olig2, NeuN, and Iba1 demonstrating astrocyte-specific activity of Aldh1l1-CreER line. n = 4 animals. Scale bars, 10 μm (b), 40 μm (e–h).
