Extended Data Fig. 3: Analysis of astrocyte development in the Gabbr1-cKO mouse line.
From: Inhibitory input directs astrocyte morphogenesis through glial GABABR
a. RNA-Scope imaging of Gabbr1 within Aldh1l1-GFP astrocytes from control and Gabbr1-cKO mouse lines; quantification derived from n = 3 pairs of animals (control: OB 18, CX 18, HC 16; cKO: OB 17, CX 19, HC 17 cells; LME model, ***P = 0.00020, ****P = 3.07e-06, **P = 0.0030). Dashed circle denotes astrocyte with Gabbr1. b. Antibody staining for SOX9 in Aldh1l1-GFP astrocytes from cortex of Gabbr1-cKO and control; quantification is derived from n = 3 pairs of animals (35 images; GLME model). c. Pulse-chase EdU-labelling and antibody staining at P28 from all brain regions analysed; quantification is derived from n = 3 pairs of animals (Gabbr1 control: OB 9, CX 9, HC 9, BS 9, CB 9; Gabbr1 cKO: OB 8, CX 9, HC 9, BS 9, CB 9 images; GLME model). d-e. Imaging of Aldh1l1-GFP astrocytes from the brain stem and cerebellum at P28; quantification of morphological complexity was derived from n = 3 pairs of animals (Gabbr1 control: BS 28, CB 29; Gabbr1 cKO: BS 32, CB 29 cells; GLME model with Sidak’s multiple comparisons test, *P = 0.0179, 0.0167). f. Quantitative analysis of branch points and process length from all brain regions analysed; n = 25-38 cells from 3 pairs of animals (Gabbr1 control: OB 38, CX 30, HC 29, BS 28, CB 25; Gabbr1 cKO: OB 33, CX 30, HC 29, BS 32, CB 29 cells; two way ANOVA, **P = 0.0014, *P = 0.0174, P = 0.9040, *P = 0.0132, P = 0.7126, **P = 0.0054, ****P < 0.0001, **P = 0.0066, P = 0.3763). Scale bars, 30 μm (d), 20 μm (c). Data represent mean ± s.d. (b-c, e-g median, minimum value, maximum value and IQR (a).
