Fig. 1: B cell response to mRNA-1273 and mRNA-1273.213 booster immunizations.
From: SARS-CoV-2 Omicron boosting induces de novo B cell response in humans
a, Seven healthy adults received an mRNA-1273 booster and thirty-nine healthy adults received a mRNA-1273.213 booster. Blood was collected at baseline and at 1, 2, 4, 8, 17 and 26 weeks after the booster. FNAs of ipsilateral axillary lymph nodes were collected 2 and 8 weeks after the booster from 5 participants from the mRNA-1273 cohort and from 20 participants from the mRNA-1273.213 cohort; bone marrow aspirates were collected 26 weeks after the booster from 3 participants from the mRNA-1273 cohort and 11 participants from the mRNA-1273.213 cohort. LN, lymph node. b, Frequencies of IgG plasmablasts (PBs) specific for the indicated antigens measured by ELISpot in week (wk) 1 PBMCs from participants who received mRNA-1273 (left; n = 7) and mRNA-1273.213 (right; n = 38). c,d, Representative flow cytometry plots of BCL6 and CD38 (c) or CD20 and CD38 (d) on IgDlo B cells in FNA (c) or PBMC (d) samples (left), pooled (WA1/2020, B.1.351, B.1.617.2 and BA.1) S protein probe (BV421 and BV650) staining on BCL6+CD38int germinal centre B cells (c) and CD20+CD38lo/int MBCs (d) (second from left), and frequencies of S+ germinal centre B cells from FNA (c) or S+ MBCs from PBMCs (d) from participants who received mRNA-1273 (second from right) and mRNA-1273.213 (right). c, mRNA-1273, n = 5; mRNA-1273.213, n = 20. d, mRNA-1273, n = 6; mRNA-1273.213, n = 28. e, Representative ELISpot wells coated with the indicated antigens, developed in blue (IgG) and red (IgA) after plating the indicated numbers of BMPCs. f, Frequencies of IgG BMPCs specific for the indicated antigens 26 weeks after participants received mRNA-1273 (left) or mRNA-1273.213 (right) boosters. Lines indicate medians. Symbols at each time point represent one sample. mRNA-1273, n = 3; mRNA-1273.213, n = 10. g,h, Binding of monoclonal antibodies from week 17 S+ MBCs from participants who received mRNA-1273 (left) and mRNA-1273.213 (right) to indicated antigens (g) and constituent domains of WA1/2020 S protein (h), measured by ELISA.
