Extended Data Fig. 1: Robust GC and Tfh responses to mRNA-1273 and mRNA-1273.213 boosters.
From: SARS-CoV-2 Omicron boosting induces de novo B cell response in humans
(a) Frequencies of IgA plasmablasts specific for the indicated antigens measured by ELISpot in week-1 PBMC from participants who received mRNA-1273 (left) and mRNA-1273.213 (right). (b) Flow cytometry plots of influenza B/HA-specific mAb 321-05 6F0534, cross-reactive mAb 382-08 1C11, and residue E484-specific mAb mu 2B0442 binding to indicated strains of SARS-CoV-2 S by multiplex bead binding array. (c) Plasma IgG binding to indicated strains of SARS-CoV-2 S measured by multiplex bead binding array in participants who received mRNA-1273 (left) and mRNA-1273.213 (right). (d) Gating strategy for analyzing S+ germinal centre B cells and T follicular helper cells (TFH) in FNA. (e) Frequencies of TFH from FNA of draining lymph nodes. (f) Correlation between frequencies of S+ germinal centre B cells and TFH. P and r values from two-sided Spearman test. (g) Representative ELISpot wells coated with BSA, and developed in blue (IgG) and red (IgA) after plating the indicated numbers of BMPCs. (h) Frequencies of IgA-secreting BMPCs specific for the indicated antigens 26 weeks post-boost. Black lines indicate medians. Symbols at each time point represent one sample. For mRNA-1273 and mRNA-1273.213 respectively, n = 7 and 38 (a), n = 6 and 28 (c), n = 5 and 20 (e), n = 3 and 10 (h).
