Extended Data Fig. 3: Subtomogram averaging pipeline.
From: In situ architecture of the ER–mitochondria encounter structure
The strategy using sequential alignment steps is outlined in steps a—h. The data set consisted of manually picked coordinates of the ER and OMM anchor points of 1098 bridge structures from 51 electron cryo-tomograms at positions of Mdm34-mNeonGreen signals. The resolution of the final STA map is potentially affected by a minor fraction of bridge-like particles of different identity, which could contribute noise. Images in a are the same as shown in Fig. 2b and c, with modifications to the overlay. Scale bars in a are 100 nm (left image) and 20 nm (images in pink dashed boxes).
