Extended Data Fig. 6: Macroscopic snowflake yeast are monoclonal, growing via permanent mother-daughter cellular bonds, not aggregation.

We co-cultured GFP and RFP-tagged genotypes of a macroscopic single strain isolate (PA2, strain ID: GOB1413-600) for 5 days, then imaged 70 clusters on a Nikon Ti-E. Shown are a composite of 11 individual clusters, which all remain entirely green or red. Individual clusters were compressed with a coverslip for imaging, resulting in their fragmentation into multiple modules. Scale bar (top-left) is 100 µm.