Extended Data Fig. 8: Cell stiffness and stress-strain curve.

a, Individual cells do not change their stiffness over 600 rounds of selection (average cell stiffness for the ancestor and t600 isolates are 0.019 and 0.020, respectively. p = 0.77, t = 0.31, df = 8, Welch’s unequal variances t-test). Single-cell stiffness values measured from atomic force microscopy (AFM) of individual cells. Error bars are one standard deviation. b, Macroscopic snowflake yeast fractured into small modules prior to compression do not show strain stiffening behaviour. Shown here is an AFM trajectory of cantilever deflection vs displacement for one t600 cluster that has been crushed into small, unentangled pieces.