Extended Data Fig. 5: Mitotic errors are associated with abnormalities in histone PTMs.
From: Epigenetic dysregulation from chromosomal transit in micronuclei
a, Representative immunofluorescence image of MDA-MB-231 cells undergoing anaphase with lagging chromosome (pointed by arrows) stained for DNA (blue) and histone PTMs (red). Scale bars 2 µm. b, Violin plots showing the normalized immunofluorescence intensity distribution of histone PTMs on normal chromosomes (NC) and lagging chromosomes (LC) of MDA-MB-231 cells during mitosis. For H3K9Me3: n = 40 NCs and LCs, for H3K27Me3: n = 42 NCs and 28 LCs, for H3K36Me3: n = 40 NCs and 31 LCs; ** p < 0.01, *** p < 0.001, **** p < 0.0001, two-sided Mann-Whitney test, solid and dashed bars in the plot represent the median and quartiles, respectively. c, Representative immunofluorescence images of MDA-MB-231 cells at different stages of the cell cycle, stained for DNA (blue) and H2BK120Ub (red), arrows point towards micronuclei, scale bars 2 µm. d, Percentage of H2BK120Ub-positive micronuclei immunofluorescence staining on vehicle (DMSO)-treated or CENP-E inhibitor (CENP-Ei, GSK923295)-treated MDA-MB-231 cells; ** p < 0.01, two-sided t-test, n = 3 biological triplicates, bars represent mean ± SD. e, Violin plot showing the fluorescence intensity quantification from EU staining experiment in MN and PN of MDA-MB-231 cells; n = 38 MN and PN, **** p < 0.0001, two-sided Mann-Whitney test. Solid and dashed bars in the plot represent the median and quartiles, respectively.
