Fig. 5: Lineage tracing showing subclonal dynamics and deterministic outgrowth.
From: Deterministic evolution and stringent selection during preneoplasia
a, Overview of prospective lineage tracing studies in TP53–/– HGOs using ECBs. The ECB parental population was split into replicates, and individual cultures evolved in parallel and subject to longitudinal barcode sequencing, showing subclonal dynamics and assessment of intrinsic or acquired fitness advantages amongst replicate cultures. b, CNA profiles were assessed by sWGS before the introduction of the ECB in the parental line and across replicate ECB cultures at multiple time points. Red asterisks denote CNAs present in at least two replicates but not in the parental population; green asterisks denote CNAs unique to one replicate. Only chromosomes harbouring newly arisen CNAs (not present in the parental population) are numbered, for simplicity. c, Muller plots depicting ECB frequencies (assessed by barcode sequencing) over time, where each colour represents a distinct subclone in each replicate. Note that, for D3C2, R2 (D3C2R2), the barcode was lost around day 273. d, Dot-plots indicating ECB subclone frequency (indicated by size) and estimated growth curve derivative per subclone (indicated by colour). Image of stomach in a is from Servier Medical Art, CC BY 3.0.
