Extended Data Fig. 8: Extra stability and protein expression results of LinearDesign-generated mRNAs encoding VZV gE protein. | Nature

Extended Data Fig. 8: Extra stability and protein expression results of LinearDesign-generated mRNAs encoding VZV gE protein.

From: Algorithm for optimized mRNA design improves stability and immunogenicity

Extended Data Fig. 8: Extra stability and protein expression results of LinearDesign-generated mRNAs encoding VZV gE protein.The alternative text for this image may have been generated using AI.

a, In-solution stability of mRNAs upon incubation in buffer (Mg2+ = 20 mM) at 37 °C. Percentage of intact mRNA is shown. Data are presented as mean ± SD from three independent experiments. b, Protein expression of mRNAs following transfection into HEK293 cells for 24 h was determined by flow cytometry. Each group has three independent assays and 10,000 live cells were collected for analysis in each assay. MFI value is presented as mean ± s.d. Kruskal–Wallis analysis of variance (ANOVA) with Dunn’s multiple comparisons test to gE-Ther group was performed for statistical analysis. *P < 0.05, ***P < 0.001.

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