Fig. 5: FNI9 mAb protects mice from seasonal IAV and IBV lethal challenges.
From: A pan-influenza antibody inhibiting neuraminidase via receptor mimicry
a–d, Percentage of body weight loss of BALB/c mice (n = 6 mice per group) prophylactically administered with murinized anti-NA FNI9 mAb (a), anti-HA stem-directed MEDI8852 mAb (b) and the same mAbs bearing the N297Q Fc mutation (c,d) 24 h before lethal infection with H1N1 A/Puerto Rico/8/34. Doses are reported in different colours and the average body weight loss for each dose group is shown. Error bars represent standard deviations. The 0% dotted line indicates baseline body weight loss; the −30% dotted line indicates body weight loss % for euthanasia based on lead veterinarian assessment as described in the ethical statement. e–h, Replicating virus titres in the lungs of BALB/c mice measured 4 days post-infection with H1N1 A/Puerto Rico/8/34 (e), H3N2 A/Singapore/INFIMH-16-0019/2016 (f) (n = 5 mice per group), B/Victoria/504/2000 (Yamagato linage) (g) and B/Brisbane/60/2008 (Victoria lineage) (h) (n = 6 mice per group) following prophylactic administration of FNI9 at 3, 0.9, 0.3 mg kg−1 (e–h) and 0.1 mg kg−1 (e,f). Each panel presents data derived from n = 1 independent experiment. Two-tailed Mann–Whitney test was used for statistical analysis of significance. LOD, limit of detection; TCID, tissue culture infectious dose.
