Fig. 1: HS promotes formation of 1:2:1:1 asymmetric FGF23–FGFR–αKlotho–HS quaternary complexes. | Nature

Fig. 1: HS promotes formation of 1:2:1:1 asymmetric FGF23–FGFR–αKlotho–HS quaternary complexes.

From: Structural basis for FGF hormone signalling

Fig. 1

Overall view of the cryo-EM reconstructions of FGF23–FGFR1c–αKlotho–HS (a), FGF23–FGFR3c–αKlotho–HS (b) and FGF23–FGFR4–αKlotho–HS (c) quaternary complexes displayed at threshold levels of 0.6, 0.45 and 0.5, respectively. The quaternary complex is shown in two different orientations related by a 180° rotation along the vertical axis. FGF23 is coloured in orange, αKlotho is shown in deep blue and HS is in magenta. Primary receptor (FGFRP) and secondary receptor (FGFRS) are shown in green and light blue in the FGF23–FGFR1c–αKlotho–HS complex, in tan and pink in the FGF23–FGFR3c–αKlotho–HS complex and in gold and light yellow in the FGF23–FGFR4–αKlotho–HS complex. Two tandem glycosidase-like domains (KL1 and KL2) and RBA of αKlotho are labelled. The relevance of the extra weak density (in grey) seen in all three 1:2:1:1 quaternary complexes is discussed in Supplementary Fig. 3.

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